The features of diagnostics of grain crop seeds intended for export

Russia occupies a leading position among the largest wheat producers and exporters. One of key requirements for biological safety domestic grain products is phytosanitary importing countries. The conformity with regard to phytopathogenic micromycetes determined by their complete absence or minimum content in batch. When shipping exported products, it necessary carry out estimation batch plant short time. In current paper there has been estimated effect proposed method sample preparation crop seeds on quality an isolated fungal pathogens’ DNA. purpose study was optimize methods extraction nucleic acids further identification reduce laboratory trials. development modern, reliable low labor-consuming diagnosing phytopathogens great urgency. order conduct research, wheat, barley rye kernels were artificially inoculated such pathogens as Parastagonospora nodorum, causative agent Septoria leaf spot, Alternaria infectoria, spot. infection carried soaking spore suspension followed incubation. assess infection, used classical mycology, well patented E.Yu. Toropova’s method. identified PCR analysis using target-specific primers. There given assessment sensitivity each studied crops. allows identifying target objects even number infected (1–5 per 195–199 healthy ones). not any related pathogens, no false-positive results during conducted analysis.